Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | activates the ceramidase activity by 2.5fold at 8-10 mol%, inhibits the ceramide synthesis activity, mechanism | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | inhibits both ceramidase and ceramide synthesis activity | Rattus norvegicus | |
C16-ceramide | ceramide synthesis activity, 10 mol%, 50% inhibition | Rattus norvegicus | |
cardiolipin | inhibits the ceramide synthesis activity: total inhibition at 2.5-5 mol%, activates the ceramidase activity, mechanism | Rattus norvegicus | |
CuCl2 | ceramide synthesis activity, 1 mM, total inhibition | Rattus norvegicus | |
dithiothreitol | inhibits both ceramidase and ceramide synthesis activity | Rattus norvegicus | |
L-erythro-sphingosine | competitive inhibitor of ceramide synthesis activity | Rattus norvegicus | |
lysophosphatidylcholine | ceramide synthesis activity, 10 mol%, moderate inhibition | Rattus norvegicus | |
additional information | the ceramide synthesis activity is not inhibited in vitro and in cells by fuminosin B1, not inhibited by EDTA or ATP, both up to 10 mM | Rattus norvegicus | |
myristaldehyde | competitively inhibits the ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition | Rattus norvegicus | |
palmitaldehyde | ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition | Rattus norvegicus | |
phosphatidic acid | ceramide synthesis activity, 2.5-5 mol%, total inhibition | Rattus norvegicus | |
phosphatidylcholine | ceramide synthesis activity, 10 mol%, moderate inhibition | Rattus norvegicus | |
phosphatidylglycerol | ceramide synthesis activity, 10 mol%, moderate inhibition | Rattus norvegicus | |
phosphatidylserine | ceramide synthesis activity, 10 mol%, moderate inhibition | Rattus norvegicus | |
sphingomyelin | ceramide synthesis activity, 5 mol%, 50% inhibition | Rattus norvegicus | |
ZnCl2 | ceramide synthesis activity, 1 mM, total inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data for the ceramide synthesis reaction, random sequential kinetic mechanism | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound, brain | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the ceramide synthesis activity is totally independent of stimulating cations | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a ceramide + H2O = a carboxylate + sphingosine | kinetic mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythro-dihydrosphingosine + fatty acid | very low ceramide synthesis activity | Rattus norvegicus | ? | - |
? | |
D-erythro-sphingosine + fatty acid | highest ceramide synthesis activity with D-erythro-sphingosine | Rattus norvegicus | ? | - |
? | |
D-threo-sphingosine + fatty acid | low ceramide synthesis activity | Rattus norvegicus | ? | - |
? | |
L-erythro-sphingosine + fatty acid | low ceramide synthesis activity | Rattus norvegicus | ? | - |
? | |
L-threo-sphingosine + fatty acid | low ceramide synthesis activity | Rattus norvegicus | ? | - |
? | |
additional information | no substrate in the ceramide synthesis reaction: palmitoyl-CoA | Rattus norvegicus | ? | - |
? | |
myristic acid + sphingosine | highest ceramide synthesis activity with myristic acid | Rattus norvegicus | N-myristoylsphingosine + H2O | - |
? | |
N-acylsphingosine + H2O | CDase cleaves the N-acyl linkage of ceramide into sphingosine and free fatty acid, brain CDase is able to catalyze the reverse reaction, i.e. ceramide synthesis | Rattus norvegicus | a carboxylate + sphingosine | - |
r | |
N-palmitoylsphingosine + H2O | - |
Rattus norvegicus | palmitic acid + sphingosine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CDase | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | ceramide synthesis activity, narrow optimum in the neutral range | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | very low ceramide synthesis activity below pH 5 or above pH 8 | Rattus norvegicus |
5.5 | 10 | broad pH-range, ceramidase activity | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | coenzyme A-independent mechanism | Rattus norvegicus |